Redalyc.Effect of sampling frequency on automatically-generated activity and freezing scores in a Pavlovian fear-conditioning preparation

نویسندگان

  • Cristina Vargas-Irwin
  • Jaime R. Robles
چکیده

Conditioned freezing has long held conceptual importance in behavior analysis, being pivotal in the explanation of anxiety-like behavior. Although initially measured indirectly, through its disruptive effect on operant behavior (conditioned suppression), and later by direct observation, automated techniques of measuring movement have recently become available, which also enable the measurement of conditioned freezing. These video processing techniques allow for the direct and virtually continuous measurement of activity, as compared to the traditional interval sampling approach of direct observation. We examined whether automatically generated freezing and movement scores were equally sensitive to traditional Pavlovian conditioning manipulations, and how this sensitivity was affected by the sampling frequency of the data. Extinction data for 42 mice were collected at a rate of 30 Hz, transformed via re-sampling and analyzed by a generalized linear model Resumen La respuesta condicionada de congelamiento tiene importancia conceptual de larga data para el Análisis Conductual, siendo clave en la explicación de las conductas de ansiedad. Aún cuando inicialmente fue medida de forma indirecta, mediante sus efectos sobre la conducta operante (como en el arreglo de supresión condicionada) y más tarde mediante la observación directa, recientemente se han hecho disponibles alternativas para la medición automática del movimiento que permiten también la medición del congelamiento condicionado. Estas nuevas técnicas de video permiten la medición directa y virtualmente constante de la actividad del organismo, por contraposición a las técnicas tradicionales de muestreo de tiempo características del registro observacional. En el presente artículo se compara el efecto de manipulaciones pavlovianas tradicionales sobre la sensibilidad de los de los índices automatizados de congelamiento y actividad, así como el posible efecto de la densidad de muestreo

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تاریخ انتشار 2009